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51.
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Fukuzawa R Fukuzawa K Abe H Nagai T Kameyama K 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2004,33(1):39-42
Abstract: A male African pygmy hedgehog ( Atelerix albiventris ), estimated to be 3 years old, presented with exophthalmos and fixed abduction of the right eye. Radiographic examination revealed a retrobulbar tumor in the right orbital cavity. The mass was surgically resected but recurred 3 months later and the hedgehog died. There was no gross or microscopic evidence of salivary or lacrimal gland involvement of the tumor at surgery or at necropsy. The histopathologic, immunohistochemical, and ultrastructural findings were those of acinic cell carcinoma, the origin of which was unknown. This is the first known case of acinic cell carcinoma in an African hedgehog. 相似文献
53.
Objective To describe a unique orbital neoplasm in dogs, of lacrimal or salivary gland origin. Animals studied Fifteen dogs with lesions consistent with a diagnosis of lobular adenomas involving the orbit were identified from the Comparative Ophthalmic Pathology Laboratory of Wisconsin from 1994 to 2001. Results The neoplasm occurred in nine females and six males. Affected dogs ranged in age from 7 to 17 years (mean = 9.7 years). Follow‐up information was available for 13 of the 15 cases. The clinical presentation included swollen/hyperemic eyelids (4/15), third eyelid protrusion (3/15), conjunctival mass (6/15), exophthalmos (4/15), resistance to retropulsion (2/15), or strabismus (1/15). In 13 cases the masses were composed of nodular, friable tissue and they were solid in two cases. Histologically, the tissue was found in encapsulated lobules resembling well differentiated lacrimal or salivary glands but completely lacking ducts. Granular PAS‐positive material was found within the cytoplasm. There was recurrence in 10 of the 13 cases available for follow‐up. Of those cases in which enucleation or exenteration was performed (3/15), there was recurrence of disease in one case. In three cases the dogs were euthanized before recurrence at 3 months, 5 months and 3 years post surgery. None of the deaths was related to the tumor. Conclusion In the 15 cases reviewed, lobular adenomas of the orbit presented clinically and histologically as a benign neoplasm of lacrimal or salivary gland origin. Recurrence was likely unless the mass was completely excised, at times requiring orbital exenteration. 相似文献
54.
镰形扇头蜱唾液腺cDNA文库的构建 总被引:4,自引:2,他引:4
构建了镰形扇头蜱唾液腺 c DNA表达文库。用 Trizol试剂提取镰形扇头蜱唾液腺总 RNA,经反转录合成 c DNA第 1链 ,应用 L D- PCR扩增方法 ,合成双链 c DNA。用 Sfi 内切酶修饰此双链 c DNA,使形成两端分别带有 Sfi A和Sfi B的粘末端。经 CHROMA SPIN- 4 0 0柱纯化 ,收集 4 0 0 bp以上的双链 c DNA片段 ,将其连接于带有 Sfi A和 Sfi B末端的λ Tripl Ex2噬菌体载体 ,经体外包装后 ,以 XL1 - Blue为受体菌构建 c DNA表达文库。经测定 ,库容量为7.0 8× 1 0 5,重组率为 95 .2 %。扩增后的文库滴度为 1 .4 5× 1 0 9pfu/ m L。随机挑取 2 0个克隆转化到质粒 p Tripl Ex2 ,经抽质粒、测序得到 2个细胞色素氧化酶全长基因 相似文献
55.
Diaz-Espiñeira MM Galac S Mol JA Rijnberk A Kooistra HS 《Domestic animal endocrinology》2008,34(2):176-181
Primary hypothyroidism in dogs is associated with increased release of growth hormone (GH). In search for an explanation we investigated the effect of intravenous administration of thyrotropin-releasing hormone (TRH, 10 microg/kg body weight) on GH release in 10 dogs with primary hypothyroidism and 6 healthy control dogs. The hypothyroid dogs had a medical history and physical changes compatible with hypothyroidism and were included in the study on the basis of the following criteria: plasma thyroxine concentration < 2 nmol/l and plasma thyrotropin (TSH) concentration > 1 microg/l. In addition, (99m)TcO(4)(-) uptake during thyroid scintigraphy was low or absent. TRH administration caused plasma TSH concentrations to rise significantly in the control dogs, but not in the hypothyroid dogs. In the dogs with primary hypothyroidism, the mean basal plasma GH concentration was relatively high (2.3+/-0.5 microg/l) and increased significantly (P=0.001) 10 and 20 min after injection of TRH (to 11.9+/-3.5 and 9.8+/-2.7 microg/l, respectively). In the control dogs, the mean basal plasma GH concentration was 1.3+/-0.1 microg/l and did not increase significantly after TRH administration. We conclude that, in contrast to healthy control dogs, primary hypothyroid dogs respond to TRH administration with a significant increase in the plasma GH concentration, possibly as a result of transdifferentiation of somatotropic pituitary cells to thyrosomatotropes. 相似文献
56.
对成年果子狸生殖腺(卵巢、睾丸)进行大体解剖和显微观察,发现其生殖腺随季节性繁殖而出现周期性变化,繁殖旺季性腺明显增大且具有丰富的各级生殖细胞;休情期则显著缩小,性腺活动处于停滞状态,没有成熟的生殖细胞。 相似文献
57.
《中国畜牧杂志》2013,(13)
本试验利用母猪行为以及唾液皮质醇和淀粉酶含量探索空间限制对母猪生理心理状态造成的影响。试验选择大白妊娠母猪64头,随机平均分成2组,分别采用限位栏(180 cm×60 cm×110 cm)和群养模式(568 cm×448 cm×50 cm)饲养,群养模式下每圈8头母猪,共4圈。于试验1、3、5、6、8、10、11、13、15周第1、2天分别采用10 min间隔扫描取样法记录母猪行为和采集试验母猪唾液样本,检测唾液皮质醇水平和唾液淀粉酶含量。结果表明:限位栏母猪表现无食咀嚼、啃栏、啃槽行为的次数显著高于群养母猪。限位栏母猪的唾液皮质醇和淀粉酶表现出相反的分泌趋势,无食咀嚼与唾液淀粉酶含量之间存在显著的正相关关系(r=0.336,P=0.048)。群养模式下试验母猪的唾液皮质醇水平、淀粉酶含量与无食咀嚼行为之间没有显著性相关的关系。 相似文献
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59.
蜱的免疫预防是目前国内外研究的热点,以抗血清为探针筛选cDNA表达文库是获取功能抗原基因的有效的方法之一,为了获得抗长角血蜱免疫原基因,用兔抗长角血蜱血清和兔抗长角血蜱唾液腺蛋白血清对长角血蜱雌蜱唾液腺cDNA表达文库进行了免疫筛选,经过两轮筛选共获得34个阳性克隆,所得阳性噬菌体转染宿主菌BM25.8使之亚克隆为重组质粒,用此质粒转化宿主菌JM109并进行序列测定和生物信息学分析.结果表明:在长角血蜱雌蜱唾液腺cDNA表达文库中获得26个长角血蜱免疫相关cDNA序列,其编码蛋白与长角血蜱HL35、黏附素hlim3假定蛋白、热休克蛋白、NADH脱氢酶、钙网蛋白以及杂色花蜱和肩突硬蜱的二硫异构酶等具有同源性.所获阳性克隆为长角血蜱保护性抗原基因的筛选奠定了基础. 相似文献
60.
用二氯甲烷作为溶剂萃取东亚飞蝗4龄、5龄以及成虫的唾液分泌物。通过GC-MS进行检测,研究发现唾液分泌物主要是2,4-二氨基[1H,3H]嘧啶二酮、棕榈酸、亚油酸、硬脂酸和胆甾醇5种成分,并且在4龄、5龄以及成虫的唾液分泌物这5种成分相同,不存在差异性。 相似文献